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Mini-Review of the Phosphate Center Activation Strategy in Nucleoside Triphosphate Preparation

[ Vol. 14 , Issue. 6 ]

Author(s):

Tomasz P. Kaczynski and Marcin K. Chmielewski*   Pages 448 - 452 ( 5 )

Abstract:


Background: Nucleoside triphosphates (NTPs) synthesis has recently been an area of searching for new methods and possible applications in biochemistry and medicine. However, we still lack a universal tool to synthesize NTPs easily and efficiently. For years, the most popular method has been the “one-pot, three step” conception by Ludwig with its subsequent improvements which used Yoshikawa's et al. monophosphorylation method. Further investigation of these reactions has proven many disadvantages: low yields, many byproducts and problems with purification. But they were a starting point for a whole branch of NTPs synthetic methods.

Method: Analysis of this evolution process demonstrates some correctness: using a pyrophosphate as a nucleophile and mechanism involving a cyclic intermediates at some stage. What is changing is the idea of phosphorus center activation and the resulting improvements and limitations.

Conclusion: In this paper we would like to discuss some new achievements in NTPs synthesis considering their role in changing the strategy of phosphorus center activation depending on the needs

Keywords:

Nucleoside triphosphates, phosphate center activation, phosphitylation, triphosphitylation, phosphoramidite, pyrophosphate.

Affiliation:

Institute of Bioorganic Chemistry, Polish Academy of Science, Noskowskiego 12-14, 61-704, Poznan, Institute of Bioorganic Chemistry, Polish Academy of Science, Noskowskiego 12-14, 61-704, Poznan

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