Tomasz P. Kaczynski and Marcin K. Chmielewski* Pages 448 - 452 ( 5 )
Background: Nucleoside triphosphates (NTPs) synthesis has recently been an area of searching for new methods and possible applications in biochemistry and medicine. However, we still lack a universal tool to synthesize NTPs easily and efficiently. For years, the most popular method has been the “one-pot, three step” conception by Ludwig with its subsequent improvements which used Yoshikawa's et al. monophosphorylation method. Further investigation of these reactions has proven many disadvantages: low yields, many byproducts and problems with purification. But they were a starting point for a whole branch of NTPs synthetic methods.
Method: Analysis of this evolution process demonstrates some correctness: using a pyrophosphate as a nucleophile and mechanism involving a cyclic intermediates at some stage. What is changing is the idea of phosphorus center activation and the resulting improvements and limitations.
Conclusion: In this paper we would like to discuss some new achievements in NTPs synthesis considering their role in changing the strategy of phosphorus center activation depending on the needs
Nucleoside triphosphates, phosphate center activation, phosphitylation, triphosphitylation, phosphoramidite, pyrophosphate.
Institute of Bioorganic Chemistry, Polish Academy of Science, Noskowskiego 12-14, 61-704, Poznan, Institute of Bioorganic Chemistry, Polish Academy of Science, Noskowskiego 12-14, 61-704, Poznan